Hamed Abdollahi

Proteomics, the large-scale study of proteins, has revolutionized our understanding of cellular processes and disease mechanisms. My expertise in this field has focused on unraveling host-pathogen protein-protein interactions, offering valuable insights into how diseases progress and how immune responses are triggered. In addition, I have extensive experience in small molecule analysis, including identifying residuals in formulated products and navigating complex downstream purification processes. My skill set includes advanced derivatization and detection techniques such as protein electrophoresis, chromatography, spectroscopy, and mass spectrometry—ensuring accurate, reliable results in even the most challenging environments.

Protein Purification

Protein purification is a crucial process in isolating a target protein from a complex biological mixture, enabling structural studies, functional characterization, and therapeutic development. My experience spans every step, from initial sample preparation through high-speed and ultra-centrifugation to advanced HPLC separation and precise quantification. My services cover all essential stages:

Sample Preparation – Efficiently handle complex biological materials.

Centrifugation & Protein Extraction – Use of high-speed and ultra-centrifugation for optimal yields.

Optimization and Purification – Tailored processes for the highest purity and activity.

Analysis and Quality Control

Protein Expression

My expertise encompasses both bacterial and yeast expression systems, each providing distinct advantages for protein production.

Bacterial Expression

Utilizing E. coli strains, bacterial expression systems offer reliable, reproducible production and purification of recombinant proteins, making them ideal for research applications.

Yeast Expression

Yeast systems are known for their high product yield and scalability. With easy genetic manipulation, yeast enables the production of proteins with complex properties that are often unattainable through bacterial cells.

Protein Electrophoresis

With extensive experience in developing, qualifying, and validating electrophoretic analytical methods, I specialize in tailoring assays to meet both client-specific needs and stringent regulatory standards. My expertise ensures precise, compliant, and high-quality solutions for a wide range of analytical challenges.

SDS-PAGE - Reducing / Non-Reducing

Isoelectric Focusing (IEF)

IPG Strips (immobilized pH gradient)

Immuno / Western Blotting

Coomassie Blue Staining

In Silico Protein Network Analysis

Protein analysis can encompass the entire protein complement of a cell, tissue, or organism under specific conditions. Since protein expression is dynamic—responding to a variety of stimuli—proteins may not always be expressed or activated when needed. As most proteins rely on interactions with other proteins for proper function, studying them within the context of their interacting partners is essential to fully understand their role. Let’s collaborate to push the boundaries of this field by combining my expertise with your resources and samples.

Genomics

While proteomics uncovers the intricacies of protein function and interaction, genomics provides the foundation by exploring DNA sequences and mutations that drive evolutionary changes and disease progression. My work in evolutionary genomics connects these two fields, studying how genetic variations translate into protein-level changes. This comprehensive approach enables the identification of biomarkers and the development of diagnostic tools, offering more accurate disease detection and personalized treatment strategies. By integrating DNA-based studies with protein analysis, I drive innovation in both research and clinical diagnostics. Key areas where genomics plays a pivotal role include:
1- Disease Research: Genomics helps identify genetic contributions to health conditions, enabling the creation of diagnostic tests, treatments, and cures that target specific genetic factors.
2- Drug & Vaccine Development: By understanding how genetics affects drug and vaccine responses, genomics supports the development of targeted therapies and personalized medicine.
3- Pharmacogenomics, in particular, explores the link between genetics and drug reactions.
4- Disease Diagnosis: Using sequencing technologies to pinpoint the genetic causes of rare or undiagnosed infectious diseases.
5- Risk Assessment: Evaluating individual risk for diseases, such as cancer, infection diseases, based on genomic variants, facilitating genetic counseling.

Nucleic Acid Extraction & Sample preparation

High-quality nucleic acid extraction, free from inhibitors and contamination, is essential for successful downstream genomic applications. I have extensive experience in supervising the extraction of RNA, mRNA, miRNA, and DNA from a variety of sources in molecular labs, including:

Animal tissues

Blood and biological fluids

FFPE samples

Limited quantities of material

Standard Genomics: PCR, ddPCR, RT-PCR, qPCR, Sanger Sequencing

With over 10 years of hands-on experience in integrated gene expression techniques—such as PCR, ddPCR ,qPCR, RT-qPCR, Sanger sequencing, DNA microarrays, and multiplex technologies—I have developed a strong skill set in troubleshooting, problem-solving, and procedural development. This expertise also extends to complex data analysis for both research and diagnostic applications.

Molecular Biology Reagents

PCR Reagents & Kits for Molecular Diagnostics

Custom, real-time qPCR, Gene expression analysis using TaqMan assays, SNP genotyping analysis and copy number variation Mutation detection

Cloning of A-tailed PCR fragments

The TA Cloning Kit with PCR offers a fast and straightforward one-step method for cloning, allowing direct insertion of PCR products into a plasmid vector. This efficient system simplifies the cloning process, making it ideal for various molecular biology applications.

Data Analysis and Interpretation

Maximize your investment in accurate, high-quality data with my comprehensive genomics solutions and bioinformatics expertise. I offer a broad range of computational services tailored to support both research and business needs, including RNA-Seq data analysis, biomarker discovery and characterization, and pathway analysis. Additionally, I can help navigate and optimize the use of commercial software, unraveling its complexities to ensure efficient and effective application in your projects.

Zoonotic Infectious Diseases

Recent advancements in molecular diagnostics have transformed how we detect and monitor infectious diseases. I bring expertise in cutting-edge molecular diagnostics, genetic analysis, and virology, with a focus on zoonotic infectious diseases, utilizing the One Health approach both in the field and laboratory settings. My expertise covers key areas such as:
1- Characterization
2- Sampling and Infectious Specimen Management
3- Biotyping and susceptibility testing
4- Molecular Diagnosis
5- Discovery, Assay Development & Validation
6- Commercializtion

Unlock High-Standard Solutions for Your Pharmaceutical, Biotech, and Academic Needs

With a depth of expertise, I can support you from early preclinical research to large-scale clinical trials for vaccines, monoclonal antibodies, and antivirals. I provide high-standard solutions in molecular diagnostics and microorganism identification—particularly zoonotics—along with virus culture, isolation, antigen testing, PCR, serotyping, and serogrouping. Trained in both BSL2 and BSL3 laboratories, I work with fully validated assays targeting highly pathogenic viruses like avian influenza. My skills extend across preclinical models, virological, molecular, cell culture, and immunological assays, ensuring accuracy and reliability at every stage. Let’s elevate your research to global standards.

With over 12 years of laboratory experience across various key sectors—including ELISA and immunoassay tests, diagnostic PCR, and the interpretation of test result reports at both the national reference laboratory level and in commercial genetically superior grandparent bird farm industries—I bring a wealth of expertise to support your laboratory's progression and ensure the highest standards of quality. I have overseen more than 300,000 immunoassays annually under my supervision, ensuring precision and reliability in diagnostics. You can rely on my expertise to help establish a comprehensive history of herd health, accurately differentiate between infections and vaccinations, and ensure your operations meet global standards for disease management and control. My expertise covers key areas such as:
1- ELISA tests
2- Virus neutralization and serum neutraliztion tests
3- Haemagglutination tests
4- Agar gel immunodiffusion (AGID) test
5- Assay Development & Validation
6- Commercializtion

Herd health and infection and vaccination history

Whether it's developing diagnostics for emerging diseases, improving herd health management, or ensuring compliance with industry regulations, I am fully equipped to guide your laboratory's efforts in advancing diagnostic precision and overall quality. Expanding on the scientific foundation of my work, I specialize in key areas such as:1- Immunoassay Development: Designing and validating robust ELISA and other immunoassays for large-scale diagnostic and research applications.2- Health Monitoring: Establishing a detailed health history and developing protocols for distinguishing between vaccine-induced immunity and active infections, crucial for effective disease management and control.

Computational Biology and Artifitial Intelligence

To convert data into actionable insights, I continuously hone my skills in state-of-the-art analytical and bioinformatics methods. By integrating molecular lab techniques with advanced bioinformatics and programming expertise, I am able to conduct cutting-edge research that improves both human and animal health through a One Health approach.

Key areas of my research in this division include:

1- Genomics and transcriptomics – Understanding gene expression, mutation impacts, and disease pathways.
2- Methylation data – Exploring epigenetic changes and their influence on health.
3- Proteomics – Investigating protein interactions and their role in diseases.
4- Protein Structure – Modeling conformational states and functional implications.

My projects and publications demonstrate a strong track record in utilizing these techniques to address complex challenges in disease research, from infectious diseases to cancer diagnostics. Through innovative bioinformatics and AI-driven approaches, I transform raw data into valuable insights, aiding advancements in diagnostics, therapeutics, and precision medicine.